The deduction is available for (below 60 years old) max  Rs. 25,000 /- to a tax payer of premium paid for Medical insurance of self, spouse and dependent children. If individual or spouse is above 60 years old or more the deduction available is Rs 30,000.
For claiming deduction, it is mandatory that the above payments should be made by any mode other than cash (e.g. cheque, credit card, debit card, etc.)

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Section 80D: Deduction for Preventive Health Check-Up
A deduction of Rs. 5000 will be allowed under this section for payment of preventive health check-up of either the individual himself or his family members which include spouse, parents and dependent children.
This deduction is not in addition to the deduction of Rs.25000/30000 stated above but is included in the above deduction.
It is important to note that Rs.5000 is the maximum total deduction allowed. This deduction is not per person but in total. So if a person pays an amount for preventive health check up for himself + spouse + dependent children + parents, the gross total deduction allowed would be Rs. 5000.
For preventive health, check-up payment can be made by any mode (including cash) for claiming the deduction.
Therefore, the maximum deduction available under this entire Section 80D is to the extent of Rs. 60,000.